Application of an in vitro endopeptidase assay for detection of residual toxin activity in tetanus toxoids.
T Leung;
PH Corran;
C Gee;
TAN Ekong;
D Sesardic;
(2002)
Application of an in vitro endopeptidase assay for detection of residual toxin activity in tetanus toxoids.
Developments in biologicals, 111.
pp. 327-332.
ISSN 1424-6074
https://material-uat.leaf.cosector.com/id/eprint/8722
Tetanus vaccine is composed of chemically denatured tetanus toxin (TeNT), thus safety testing requires confirmation of freedom from residual and reversible toxicity. Currently, TeNT activity is estimated using in vivo assay models. Information that TeNT acts by selectively inactivating protein leading to the blocking of release of neurotransmitters has provided the opportunity to develop in vitro biochemical assay for toxin activity. In this study we describe development and use of an in vitro endopeptidase assay for detection of TeNT activity in toxoid vaccine formulations.
| Item Type | Article |
|---|---|
| Keywords | Animal Testing Alternatives, Biological Assay, Endopeptidases/*metabolism, Membrane Proteins/genetics/metabolism, Nerve Tissue Proteins/genetics/metabolism, R-SNARE Proteins, Tetanus Toxin/*analysis/immunology/toxicity, Tetanus Toxoid/*chemistry/toxicity, Toxicity Tests/*methods, Animal Testing Alternatives, Biological Assay, Endopeptidases, metabolism, Membrane Proteins, genetics, metabolism, Nerve Tissue Proteins, genetics, metabolism, R-SNARE Proteins, Tetanus Toxin, analysis, immunology, toxicity, Tetanus Toxoid, chemistry, toxicity, Toxicity Tests, methods |