Etiology of severe childhood pneumonia in the Gambia, West Africa, determined by conventional and molecular microbiological analyses of lung and pleural aspirate samples.

Stephen RC Howie; Gerard AJ Morris; Rafal Tokarz; Bernard E Ebruke; Eunice M Machuka; Readon C Ideh; Osaretin Chimah; Ousman Secka; John Townend; Michel Dione; +11 more... Claire Oluwalana; Malick Njie; Mariatou Jallow; Philip C Hill; Martin Antonio ORCID logo; Brian Greenwood ORCID logo; Thomas Briese; Kim Mulholland ORCID logo; Tumani Corrah; W Ian Lipkin; Richard A Adegbola; (2014) Etiology of severe childhood pneumonia in the Gambia, West Africa, determined by conventional and molecular microbiological analyses of lung and pleural aspirate samples. Clinical infectious diseases, 59 (5). pp. 682-685. ISSN 1058-4838 DOI: 10.1093/cid/ciu384
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Molecular analyses of lung aspirates from Gambian children with severe pneumonia detected pathogens more frequently than did culture and showed a predominance of bacteria, principally Streptococcus pneumoniae, >75% being of serotypes covered by current pneumococcal conjugate vaccines. Multiple pathogens were detected frequently, notably Haemophilus influenzae (mostly nontypeable) together with S. pneumoniae.


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