Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP.
C Fanello;
F Santolamazza;
A della Torre;
(2002)
Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP.
Medical and veterinary entomology, 16 (4).
pp. 461-464.
ISSN 0269-283X
DOI: 10.1046/j.1365-2915.2002.00393.x
For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto, we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large-scale processing of field-collected larval and adult specimens to be identified in malaria vector studies.
| Item Type | Article |
|---|---|
| Keywords | Africa, Aging, Animal, Anopheles/*classification/*genetics, Base Sequence, *Genes, Insect, Larva/classification/genetics, *Polymerase Chain Reaction, *Polymorphism, Restriction Fragment Length, Species Specificity, Support, Non-U.S. Gov't |
| ISI | 179803600018 |