Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12.

Janine T Bossé; Yanwen Li; Øystein Angen; Lucy A Weinert; Roy R Chaudhuri; Matthew T Holden; Susanna M Williamson; Duncan J Maskell; Alexander W Tucker; Brendan W Wren

; +3 more... Andrew N Rycroft; Paul R Langford; BRaDP1T consortium; (2014) Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12. Journal of clinical microbiology, 52 (7). pp. 2380-2385. ISSN 0095-1137 DOI: 10.1128/JCM.00685-14

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An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.