Comparison of a new, affordable flow cytometric method and the manual magnetic bead technique for CD4 T-lymphocyte counting in a northern Nigerian setting.

Godwin E Imade; Bitrus Badung; Sunday Pam; Oche Agbaji; Daniel Egah; Atiene S Sagay; Jean-Louis Sankalé; Saidi Kapiga ORCID logo; John Idoko; Phyllis Kanki; (2005) Comparison of a new, affordable flow cytometric method and the manual magnetic bead technique for CD4 T-lymphocyte counting in a northern Nigerian setting. Clinical and diagnostic laboratory immunology, 12 (1). pp. 224-227. ISSN 1071-412X DOI: 10.1128/CDLI.12.1.224-227.2005
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We compared two techniques for CD4 T-lymphocyte counting: flow cytometry (Cyflow) and magnetic beads (Dynabead). Similar results with good correlation were obtained from the 40 adult blood samples counted (P=0.057, r=0.93). The Cyflow technique is more precise and cost-effective than the Dynabead method ($3 to $5 versus $12 to $22 per test, respectively), since as many as 200 samples can be measured per day.

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