Dynamic variation in the cellular origin of HIV type 1 during treatment of tuberculosis in dually infected subjects.
HIV-1 replication remains elevated in dually infected HIV-1/TB subjects at completion of antituberculosis therapy. A viral immunocapture assay was used to examine the cellular origin of HIV-1 within plasma from HIV-1/TB subjects at time of diagnosis of pulmonary TB, at end of TB treatment, and 6 months after completion of treatment. Asymptomatic HIV-1-infected subjects without TB (HIV-1/C) served as controls. Both activated immature macrophage (CD36(+)) and CD4 T cell (CD26(+)) compartments contributed to viral load. Changes in the activation status of either cellular compartment paralleled their contribution to viral load. Levels of HIV-1 originating from activated (HLA-DR(+)) cells and from CD36(+) and CD26(+) mononuclear cells resolved to levels observed in HIV-1/C by the end of treatment. HIV-1 isolated by anti-CD3 immunocapture from HIV-1/TB patients remained significantly higher than from HIV-1/C patients at the end of TB treatment and at 12 months follow-up. Therefore, viral production by lymphocytes extends well beyond the completion of TB treatment.
| Item Type | Article |
|---|---|
| Keywords | IMMUNODEFICIENCY-VIRUS TYPE-1, PULMONARY TUBERCULOSIS, IMMUNE, ACTIVATION, MYCOBACTERIUM-TUBERCULOSIS, OPPORTUNISTIC INFECTION, IN-VIVO, REPLICATION, MACROPHAGES, PROTEINS, IMPACT, Adolescent, Adult, Antigens, CD3, immunology, CD4 Lymphocyte Count, CD4-Positive T-Lymphocytes, immunology, virology, Comorbidity, Female, Flow Cytometry, Follow-Up Studies, HIV Infections, complications, immunology, virology, HIV-1, physiology, HLA-DR Antigens, analysis, Humans, Immunohistochemistry, Leukocytes, Mononuclear, cytology, virology, Male, Prospective Studies, RNA, Viral, blood, Time Factors, Treatment Outcome, Tuberculosis, Pulmonary, complications, diagnosis, drug therapy, immunology, microbiology, radiography, Viral Load |
| ISI | 243995300013 |