Analysis of protection afforded by a Clostridium perfringens alpha-toxoid against heterologous clostridial phospholipases C.
The major virulence determinant in clostridial myonecrosis caused by Clostridium perfringens is a phospholipase C (PLC), the alpha-toxin. Previously, mice have been protected against challenge with heterologous alpha-toxin or Clostridium perfringens spores by immunisation with the C-domain (known as Cpa(247-370) or alpha-toxoid) of the alpha-toxin. In this study, we have determined the ability of the alpha-toxoid to protect against the lethal effects of a divergent C. perfringens alpha-toxin and against the PLCs of C. absonum or C. bifermentans, species which have been isolated from cases of clostridial myonecrosis. Protection against the C. perfringens alpha-toxin variant, the C. absonum alpha-toxin or the C. bifermentans PLC was elicited by immunisation with the alpha-toxoid in vivo.
| Item Type | Article |
|---|---|
| Keywords | Animals, Bacterial Toxins, immunology, Bacterial Vaccines, immunology, pharmacology, Calcium-Binding Proteins, immunology, Cattle, Clostridium Infections, enzymology, immunology, microbiology, prevention & control, Clostridium bifermentans, enzymology, Clostridium perfringens, immunology, Cross Reactions, Female, Mice, Mice, Inbred BALB C, Recombinant Proteins, biosynthesis, genetics, isolation & purification, pharmacology, Type C Phospholipases, antagonists & inhibitors, immunology, metabolism |
| ISI | 249271300003 |