Screening for HIV-specific T-cell responses using overlapping 15-mer peptide pools or optimized epitopes.
Tara Beattie ;
RupertKaul;
TimRostron;
TaoDong;
PhilippaEasterbrook;
WalterJaoko;
JoshuaKimani;
FrancisPlummer;
AndrewMcMichael;
SarahRowland-Jones;
(2004)
Screening for HIV-specific T-cell responses using overlapping 15-mer peptide pools or optimized epitopes.
AIDS (London, England), 18 (11).
pp. 1595-1598.
ISSN 0269-9370
DOI: 10.1097/01.aids.0000131362.82951.b2
The IFN-y enzyme-linked immunospot (ELI-Spot) assay is often used to map HIV-specific CD8 T-cell responses. We compared overlapping 15-mer pools with optimized CD8 epitopes to screen ELISpot responses in HIV-infected individuals. The 15-mer pools detected responses to previously undefined epitopes, but often missed low-level responses to predefined epitopes, particularly when the epitope was central in the 15-mer, rather than at the N-terminus or C-terminus. These factors should be considered in the monitoring of HIV vaccine trials.
Item Type | Article |
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ISI | 222934500015 |
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ORCID: https://orcid.org/0000-0001-9156-7341