Use of commercial enzyme immunoassays to detect antibodies to the herpes simplex virus type 2 glycoprotein G in a low-risk population in Hanoi, Vietnam.

Thoai D Ngo; Oliver Laeyendecker; Hanh La; Wayne Hogrefe; Rhoda Ashley Morrow; Thomas C Quinn; (2008) Use of commercial enzyme immunoassays to detect antibodies to the herpes simplex virus type 2 glycoprotein G in a low-risk population in Hanoi, Vietnam. Clinical and vaccine immunology, 15 (2). pp. 382-384. ISSN 1556-6811 DOI: 10.1128/CVI.00437-06
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Sera from 1,238 Vietnamese women in Hanoi were tested for herpes simplex virus type 2 (HSV-2). HSV-2 prevalence was 2.0%. The Kalon and Biokit assays showed significantly higher concordance to Western blotting data than did the Focus assay (P < 0.01). Screening by Focus and then retesting with Kalon/Biokit of positive samples can reduce falsely positive results significantly (P < 0.01).

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