Evaluation of a PCR-based universal heteroduplex generator assay as a tool for rapid detection of multidrug-resistant Mycobacterium tuberculosis in Peru.
Holger Mayta;
Robert H Gilman;
Fanny Arenas;
Teresa Valencia;
Luz Caviedes;
Sonia H Montenegro;
Eduardo Ticona;
Jaime Ortiz;
Rosa Chumpitaz;
Carlton A Evans;
+2 more...
Diana L Williams;
Tuberculosis Working Group in Perú;
(2003)
Evaluation of a PCR-based universal heteroduplex generator assay as a tool for rapid detection of multidrug-resistant Mycobacterium tuberculosis in Peru.
Journal of clinical microbiology, 41 (12).
pp. 5774-5777.
ISSN 0095-1137
DOI: 10.1128/JCM.41.12.5774-5777.2003
Multidrug-resistant tuberculosis is an increasing health problem worldwide, especially in developing countries. The PCR-UHG-Rif assay, which detects mutations within the rpoB gene associated with rifampin resistance, was evaluated for its ability and reliability to detect and identify drug-resistant Mycobacterium tuberculosis in a developing country where tuberculosis is highly endemic.
| Item Type | Article |
|---|---|
| Keywords | Antitubercular Agents/pharmacology/therapeutic use, DNA-Directed RNA Polymerases/genetics, Drug Resistance, Multiple/*genetics, Humans, Microbial Sensitivity Tests, Mycobacterium tuberculosis/drug effects/*genetics/isolation & purification, Peru, Polymerase Chain Reaction/*methods, Predictive Value of Tests, Rifampin/pharmacology/therapeutic use, Sensitivity and Specificity, Tuberculosis/drug therapy |
| ISI | 187228800070 |