Transcriptome of enterohemorrhagic Escherichia coli O157 adhering to eukaryotic plasma membranes.

Sivan Dahan; Stuart Knutton; Robert K Shaw; Valerie F Crepin; Gordon Dougan; Gad Frankel; (2004) Transcriptome of enterohemorrhagic Escherichia coli O157 adhering to eukaryotic plasma membranes. Infection and immunity, 72 (9). pp. 5452-5459. ISSN 0019-9567 DOI: 10.1128/IAI.72.9.5452-5459.2004
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Using a DNA microarray, we determined changes in enterohemorrhagic Escherichia coli O157:H7 gene expression during binding to plasma membranes. Analysis of the complete transcriptomes of the bound bacteria revealed increased levels of stress-associated mRNAs and decreased levels of mRNA encoding proteins involved in translation and type III secretion.

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