Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men.
Andrew Birtles;
Noel McCarthy;
Carmen L Sheppard;
Harry Rutter;
Malcolm Guiver;
Elizabeth Haworth;
Robert C George;
(2005)
Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men.
Journal of clinical microbiology, 43 (4).
pp. 2004-2008.
ISSN 0095-1137
DOI: 10.1128/JCM.43.4.2004-2008.2005
Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.
| Item Type | Article |
|---|---|
| Keywords | Adult, Bacterial Proteins/*genetics, Bacterial Typing Techniques, Blood/microbiology, Cerebrospinal Fluid/microbiology, Fatal Outcome, *Homeless Persons, Humans, Male, Meningitis, Pneumococcal/*microbiology, Polymerase Chain Reaction, *Sequence Analysis, DNA, Streptococcus pneumoniae/*classification/genetics/isolation & purification, Streptolysins/*genetics, Adult, Bacterial Proteins, genetics, Bacterial Typing Techniques, Blood, microbiology, Cerebrospinal Fluid, microbiology, Fatal Outcome, Homeless Persons, Humans, Male, Meningitis, Pneumococcal, microbiology, Polymerase Chain Reaction, Sequence Analysis, DNA, Streptococcus pneumoniae, classification, genetics, isolation & purification, Streptolysins, genetics |
| ISI | 228404100088 |
Explore Further
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1081336 (OA Location)
- 10.1128/JCM.43.4.2004-2008.2005 (DOI)
- 15815045 (PubMed)