Genetic characterisation of uninucleated cyst-producing Entamoeba spp. from ruminants.
C Rune Stensvold;
Marianne Lebbad;
C Graham Clark 
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(2010)
Genetic characterisation of uninucleated cyst-producing Entamoeba spp. from ruminants.
International journal for parasitology, 40 (7).
pp. 775-778.
ISSN 0020-7519
DOI: 10.1016/j.ijpara.2010.03.003
;
(2010)
Genetic characterisation of uninucleated cyst-producing Entamoeba spp. from ruminants.
International journal for parasitology, 40 (7).
pp. 775-778.
ISSN 0020-7519
DOI: 10.1016/j.ijpara.2010.03.003
Six ssrRNA gene sequences were obtained by PCR amplification of DNA from uninucleated Entamoeba cysts isolated from fresh faeces of sheep, cows, a roe deer and a reindeer. Phylogenetic analysis using sequences of non-, uni-, quadri- and octonucleate cyst-producing Entamoeba spp. for comparison showed that all six isolates formed a separate clade nested within the clade of quadrinucleate cyst producers. The data indicate that Entamoeba bovis can be isolated from ruminant hosts other than cattle, and we suggest that organisms clustering with the sheep and cattle isolates analysed in the present study be named E. bovis.
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