Inter- and intra-assay reproducibility of microplate Alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis.

Brian Leonard; Jorge Coronel; Mark Siedner; Louis Grandjean; Luz Caviedes; Pilar Navarro; Robert H Gilman; David AJ Moore

; (2008) Inter- and intra-assay reproducibility of microplate Alamar blue assay results for isoniazid, rifampicin, ethambutol, streptomycin, ciprofloxacin, and capreomycin drug susceptibility testing of Mycobacterium tuberculosis. Journal of clinical microbiology, 46 (10). pp. 3526-3529. ISSN 0095-1137 DOI: 10.1128/JCM.02083-07

Copy

The intersample and intrasample variability of the results obtained with the microplate Alamar blue assay for the indirect drug susceptibility testing of Mycobacterium tuberculosis was investigated. Between 1.2 and 8.5% of paired MICs differed by more than one twofold dilution, resulting in discordant susceptible-resistant designations at frequencies between 0.6% (rifampin) and 18.9% (ethambutol).

Item Type	Article
Keywords	Antitubercular Agents/pharmacology, Humans, Microbial Sensitivity Tests/methods, Mycobacterium tuberculosis/drug effects, Oxazines/metabolism, Reproducibility of Results, Xanthenes/metabolism, Antitubercular Agents, pharmacology, Humans, Microbial Sensitivity Tests, methods, Mycobacterium tuberculosis, drug effects, Oxazines, metabolism, Reproducibility of Results, Xanthenes, metabolism
ISI	259758900060