Making standards for quantitative real-time pneumococcal PCR.
Susan C Morpeth;
Jim F Huggett;
David R Murdoch;
J Anthony G Scott 
;
(2014)
Making standards for quantitative real-time pneumococcal PCR.
Biomolecular detection and quantification, 2 (C).
pp. 1-3.
ISSN 2214-7535
DOI: 10.1016/j.bdq.2014.11.003
;
(2014)
Making standards for quantitative real-time pneumococcal PCR.
Biomolecular detection and quantification, 2 (C).
pp. 1-3.
ISSN 2214-7535
DOI: 10.1016/j.bdq.2014.11.003
Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1-1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantification.
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