Two distinct broadly neutralizing antibody specificities of different clonal lineages in a single HIV-1-infected donor: implications for vaccine design.

Mattia Bonsignori; David C Montefiori; Xueling Wu; Xi Chen; Kwan-Ki Hwang; Chun-Yen Tsao; Daniel M Kozink; Robert J Parks; Georgia D Tomaras; John A Crump; +7 more... Saidi H Kapiga

; Noel E Sam; Peter D Kwong; Thomas B Kepler; Hua-Xin Liao; John R Mascola; Barton F Haynes; (2012) Two distinct broadly neutralizing antibody specificities of different clonal lineages in a single HIV-1-infected donor: implications for vaccine design. Journal of virology, 86 (8). pp. 4688-4692. ISSN 0022-538X DOI: 10.1128/JVI.07163-11

Copy

Plasma from a small subset of subjects chronically infected with HIV-1 shows remarkable magnitude and breadth of neutralizing activity. From one of these individuals (CH0219), we isolated two broadly neutralizing antibodies (bnAbs), CH01 and VRC-CH31, from two clonal lineages of memory B cells with distinct specificities (variable loop 1 and 2 [V1V2] conformational specificity and CD4-binding site specificity, respectively) that recapitulate 95% of CH0219 serum neutralization breadth. These data provide proof of concept for an HIV-1 vaccine that aims to elicit bnAbs of multiple specificities.

Item Type	Article
Keywords	immunodeficiency-virus type-1, human monoclonal-antibody, hiv-antibodies, epitope, glycoprotein, individuals, breadth, reveal, gp41, pg16, AIDS Vaccines, immunology, Antibodies, Neutralizing, genetics, immunology, metabolism, Antibody Specificity, immunology, Antigens, CD4, chemistry, metabolism, B-Lymphocytes, immunology, Binding Sites, Epitopes, chemistry, HIV Antibodies, genetics, immunology, metabolism, HIV Envelope Protein gp120, chemistry, immunology, HIV Infections, immunology, HIV-1, immunology, Humans, Immunoglobulin Variable Region, chemistry, genetics, Neutralization Tests, Phylogeny
ISI	302185400062