Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage.

International Stem Cell Initiative; KatherineAmps; Peter WAndrews; GeorgeAnyfantis; LyleArmstrong; StuartAvery; HosseinBaharvand; JulieBaker; DuncanBaker; Maria BMunoz; +116 more... StephenBeil; NissimBenvenisty; DalitBen-Yosef; Juan-CarlosBiancotti; AlexisBosman; Romulo MartinBrena; DanielBrison; GunillaCaisander; María VCamarasa; JiemingChen; EricChiao; Young MinChoi; Andre BHChoo; DanielCollins; AlanColman; Jeremy MCrook; George QDaley; AnneDalton; Paul ADe Sousa; ChrisDenning; JanetDownie; PetrDvorak; Karen DMontgomery; AnisFeki; AngelaFord; VictoriaFox; Ana MFraga; TzviaFrumkin; LinGe; Paul JGokhale; TamarGolan-Lev; HamidGourabi; MichalGropp; GuangxiuLu; AlesHampl; KatieHarron; LynHealy; WishvaHerath; FridaHolm; OutiHovatta; JohanHyllner; Maneesha SInamdar; Astrid KresentiaIrwanto; TetsuyaIshii; MarisaJaconi; YingJin; SusanKimber; SergeyKiselev; Barbara BKnowles; OdedKopper; ValeriKukharenko; AnverKuliev; Maria ALagarkova; Peter WLaird; MajlindaLako; Andrew LLaslett; NetaLavon; Dong RyulLee; Jeoung EunLee; ChunliangLi; Linda SLim; Tenneille ELudwig; YuMa; EdnaMaltby; IleanaMateizel; YoavMayshar; MariaMileikovsky; Stephen LMinger; TakamichiMiyazaki; Shin YongMoon; HarryMoore; ChristineMummery; AndrasNagy; NorioNakatsuji; KavitaNarwani; Steve KWOh; Sun KyungOh; CiaOlson; TimoOtonkoski; FeiPan; In-HyunPark; StevePells; Martin FPera; Lygia VPereira; OuyangQi; Grace SelvaRaj; BenjaminReubinoff; AlanRobins; PaulRobson; JanetRossant; Ghasem HSalekdeh; Thomas CSchulz; KarenSermon; JameelahSheik Mohamed; HuiShen; EricSherrer; KuldipSidhu; ShiraniSivarajah; HeliSkottman; ClaudiaSpits; Glyn NStacey; RaimundStrehl; NickStrelchenko; HirofumiSuemori; BowenSun; RiittaSuuronen; KazutoshiTakahashi; TimoTuuri; ParvathyVenu; YuriVerlinsky; DorienWard-van Oostwaard; Daniel JWeisenberger; YueWu; ShinyaYamanaka; LorraineYoung; QiZhou; (2011) Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage. Nature biotechnology, 29 (12). pp. 1132-1144. ISSN 1087-0156 DOI: 10.1038/nbt.2051
Copy

The International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell lines and 11 induced pluripotent stem (iPS) cell lines, from 38 laboratories worldwide, for genetic changes occurring during culture. Most lines were analyzed at an early and late passage. Single-nucleotide polymorphism (SNP) analysis revealed that they included representatives of most major ethnic groups. Most lines remained karyotypically normal, but there was a progressive tendency to acquire changes on prolonged culture, commonly affecting chromosomes 1, 12, 17 and 20. DNA methylation patterns changed haphazardly with no link to time in culture. Structural variants, determined from the SNP arrays, also appeared sporadically. No common variants related to culture were observed on chromosomes 1, 12 and 17, but a minimal amplicon in chromosome 20q11.21, including three genes expressed in human ES cells, ID1, BCL2L1 and HM13, occurred in >20% of the lines. Of these genes, BCL2L1 is a strong candidate for driving culture adaptation of ES cells.
Item Type Article
ISI 298038700025

Atom BibTeX OpenURL ContextObject in Span Multiline CSV OpenURL ContextObject Dublin Core Dublin Core MPEG-21 DIDL EndNote HTML Citation JSON MARC (ASCII) MARC (ISO 2709) METS MODS RDF+N3 RDF+N-Triples RDF+XML RIOXX2 XML Reference Manager Refer Simple Metadata ASCII Citation EP3 XML
Export

Downloads

Full text not available from this repository.

Explore Further

Read more research from the creator(s):

Find work associated with the faculties and division(s):

Find work from this publication:

Find other related resources: