Prolonged measles virus shedding in human immunodeficiency virus-infected children, detected by reverse transcriptase-polymerase chain reaction.
A reverse transcriptase-polymerase chain reaction assay was used to detect measles virus RNA in peripheral blood mononuclear cells, urine, and nasopharyngeal specimens from Zambian children during hospitalization and approximately 1-2 months after discharge. Of 47 children, 29 (61.7%) had prolonged measles virus shedding, as defined by detection of measles virus RNA in > or =1 specimen obtained 30-61 days after rash onset. Ten (90.9%) of 11 human immunodeficiency virus (HIV)-infected children had prolonged measles virus shedding, compared with 19 (52.8%) of 36 HIV-uninfected children (P=.02). Prolonged measles virus shedding did not correlate with levels of measles virus-specific antibody. HIV-infected children with measles may have a prolonged infectious period that potentially enhances measles virus transmission and hinders measles control.
| Item Type | Article |
|---|---|
| Keywords | Dependent cellular cytotoxicity, immune-responses, clinical-, samples, antibody, mortality, viremia, antigen, adults, cells, rna, Adolescence, Antibodies, Viral, blood, Child, Child, Preschool, Female, HIV Infections, complications, HIV Seronegativity, HIV-1, isolation & purification, Human, Infant, Leukocytes, Mononuclear, virology, Male, Measles, complications, virology, Measles virus, genetics, immunology, isolation & purification, physiology, Nasopharynx, virology, RNA, Viral, analysis, Reverse Transcriptase Polymerase Chain Reaction, Support, Non-U.S. Gov't, Support, U.S. Gov't, P.H.S., Time Factors, Urine, virology, Virus Shedding, physiology, Zambia |
| ISI | 166586000002 |