Use of a rapid, single-round, multiplex PCR to detect malarial parasites and identify the species present.
D Padley;
AH Moody;
PL Chiodini;
J Saldanha;
(2003)
Use of a rapid, single-round, multiplex PCR to detect malarial parasites and identify the species present.
Annals of tropical medicine and parasitology, 97 (2).
pp. 131-137.
ISSN 0003-4983
DOI: 10.1179/000349803125002977
A new, rapid assay, based on a single-round, multiplex PCR, can be used to detect Plasmodium falciparum, P. vivax, P. malariae or P. ovale in human blood. The PCR, which targets the conserved 18S small-subunit RNA genes of the parasites, not only permits a malarial infection to be detected but also allows each Plasmodium species present to be identified, even in cases of mixed infection.
| Item Type | Article |
|---|---|
| Keywords | Animal, DNA, Protozoan/analysis, Human, Malaria/*diagnosis/parasitology, Parasitemia/*diagnosis/parasitology, Plasmodium/*isolation & purification, Plasmodium falciparum/isolation & purification, Plasmodium malariae/isolation & purification, Plasmodium ovale/isolation & purification, Plasmodium vivax/isolation & purification, Polymerase Chain Reaction/*methods, Sensitivity and Specificity, Animals, DNA, Protozoan, analysis, Human, Malaria, diagnosis, parasitology, Parasitemia, diagnosis, parasitology, Plasmodium, isolation & purification, Plasmodium falciparum, isolation & purification, Plasmodium malariae, isolation & purification, Plasmodium ovale, isolation & purification, Plasmodium vivax, isolation & purification, Polymerase Chain Reaction, methods, Sensitivity and Specificity |
| ISI | 182598400004 |