Genetic marker suitable for identification and genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri.

Naowarat Tanomsing; Mallika Imwong; Colin J Sutherland ORCID logo; Christiane Dolecek; Tran Tinh Hien; Francois Nosten; Nicholas PJ Day; Nicholas J White; Georges Snounou; (2013) Genetic marker suitable for identification and genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri. Journal of clinical microbiology, 51 (12). pp. 4213-4216. ISSN 0095-1137 DOI: 10.1128/JCM.01527-13
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We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimination of intraspecific sequence variants at this locus.


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