In-house phage amplification assay is a sound alternative for detecting rifampin-resistant Mycobacterium tuberculosis in low-resource settings.

Norberto Símboli; Howard Takiff; Ruth McNerney; Beatriz López; Anandi Martin; Juan Carlos Palomino; Lucía Barrera; Viviana Ritacco; (2005) In-house phage amplification assay is a sound alternative for detecting rifampin-resistant Mycobacterium tuberculosis in low-resource settings. Antimicrobial agents and chemotherapy, 49 (1). pp. 425-427. ISSN 0066-4804 DOI: 10.1128/AAC.49.1.425-427.2005

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An in-house mycobacteriophage amplification assay for detecting rifampin-resistant Mycobacterium tuberculosis showed 100% sensitivity, 97.7% specificity, and 95.2% predictive value for resistance in a test of 129 isolates from a hot spot area of multidrug-resistant M. tuberculosis. The applicability of the test was demonstrated in the routine work flow of a low-resource reference laboratory.