Construction of varicella-zoster virus recombinants from parent Oka cosmids and demonstration that ORF65 protein is dispensable for infection of human skin and T cells in the SCID-hu mouse model.
Takahiro Niizuma;
Leigh Zerboni;
Marvin H Sommer;
Hideki Ito;
Stewart Hinchliffe;
Ann M Arvin;
(2003)
Construction of varicella-zoster virus recombinants from parent Oka cosmids and demonstration that ORF65 protein is dispensable for infection of human skin and T cells in the SCID-hu mouse model.
Journal of virology, 77 (10).
pp. 6062-6065.
ISSN 0022-538X
DOI: 10.1128/jvi.77.10.6062-6065.2003
We generated an ORF65 deletion mutant by using a cosmid system constructed from the genome of a low-passage clinical isolate (P-Oka). Using the SCID-hu mouse model, we demonstrated that the ORF65 protein is dispensable for viral replication in skin and T cells, which are critical host cell targets during primary varicella-zoster virus infection.
| Item Type | Article |
|---|---|
| ISI | 182631100052 |