The genome of the kinetoplastid parasite, Leishmania major.

Alasdair C Ivens; Christopher S Peacock; Elizabeth A Worthey; Lee Murphy; Gautam Aggarwal; Matthew Berriman; Ellen Sisk; Marie-Adele Rajandream; Ellen Adlem; Rita Aert; +91 more... Atashi Anupama; Zina Apostolou; Philip Attipoe; Nathalie Bason; Christopher Bauser; Alfred Beck; Stephen M Beverley; Gabriella Bianchettin; Katja Borzym; Gordana Bothe; Carlo V Bruschi; Matt Collins; Eithon Cadag; Laura Ciarloni; Christine Clayton; Richard MR Coulson; Ann Cronin; Angela K Cruz; Robert M Davies; Javier De Gaudenzi; Deborah E Dobson; Andreas Duesterhoeft; Gholam Fazelina; Nigel Fosker; Alberto Carlos Frasch; Audrey Fraser; Monika Fuchs; Claudia Gabel; Arlette Goble; André Goffeau; David Harris; Christiane Hertz-Fowler; Helmut Hilbert; David Horn; Yiting Huang; Sven Klages; Andrew Knights; Michael Kube; Natasha Larke; Lyudmila Litvin; Angela Lord; Tin Louie; Marco Marra; David Masuy; Keith Matthews; Shulamit Michaeli; Jeremy C Mottram; Silke Müller-Auer; Heather Munden; Siri Nelson; Halina Norbertczak; Karen Oliver; Susan O'neil; Martin Pentony; Thomas M Pohl; Claire Price; Bénédicte Purnelle; Michael A Quail; Ester Rabbinowitsch; Richard Reinhardt; Michael Rieger; Joel Rinta; Johan Robben; Laura Robertson; Jeronimo C Ruiz; Simon Rutter; David Saunders; Melanie Schäfer; Jacquie Schein; David C Schwartz; Kathy Seeger; Amber Seyler; Sarah Sharp; Heesun Shin; Dhileep Sivam; Rob Squares; Steve Squares; Valentina Tosato; Christy Vogt; Guido Volckaert; Rolf Wambutt; Tim Warren; Holger Wedler; John Woodward; Shiguo Zhou; Wolfgang Zimmermann; Deborah F Smith; Jenefer M Blackwell; Kenneth D Stuart; Bart Barrell; Peter J Myler; (2005) The genome of the kinetoplastid parasite, Leishmania major. Science (New York, NY), 309 (5733). pp. 436-442. ISSN 0036-8075 DOI: 10.1126/science.1112680

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Leishmania species cause a spectrum of human diseases in tropical and subtropical regions of the world. We have sequenced the 36 chromosomes of the 32.8-megabase haploid genome of Leishmania major (Friedlin strain) and predict 911 RNA genes, 39 pseudogenes, and 8272 protein-coding genes, of which 36% can be ascribed a putative function. These include genes involved in host-pathogen interactions, such as proteolytic enzymes, and extensive machinery for synthesis of complex surface glycoconjugates. The organization of protein-coding genes into long, strand-specific, polycistronic clusters and lack of general transcription factors in the L. major, Trypanosoma brucei, and Trypanosoma cruzi (Tritryp) genomes suggest that the mechanisms regulating RNA polymerase II-directed transcription are distinct from those operating in other eukaryotes, although the trypanosomatids appear capable of chromatin remodeling. Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression.

Item Type	Article
Keywords	Animals, Chromatin, genetics, metabolism, Gene Expression Regulation, Genes, Protozoan, Genes, rRNA, Genome, Protozoan, Glycoconjugates, biosynthesis, metabolism, Leishmania major, chemistry, genetics, metabolism, Leishmaniasis, Cutaneous, parasitology, Lipids, metabolism, Membrane Proteins, biosynthesis, chemistry, genetics, metabolism, Molecular Sequence Data, Multigene Family, Protein Biosynthesis, Protein Processing, Post-Translational, Protozoan Proteins, biosynthesis, chemistry, genetics, metabolism, RNA Processing, Post-Transcriptional, RNA Splicing, RNA, Protozoan, genetics, metabolism, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S., Sequence Analysis, DNA, Transcription, Genetic
ISI	230574900038